Epigenomic Regulation of HTLV Replication & Pathogenesis
- CTCF is a chromatin barrier element
- Single CTCF binding site in HTLV-1 DNA
- Regulates virus expression and DNA looping
- Loss of CTCF binding attenuate pathogenesis
HTLV Integration Preference & Lymphomagenesis
- Role of Protein Phosphatase 2A in HTLV-1 IN binding, virus replication and T-cell immortalization
- Using virus with mutation in binding sites and cells with CRISPR/Cas9 deletion
Role of T-Cell Receptor Signaling in HTLV-Leukemia/Lymphoma
- Frequent gain-of-function mutations in TCR pathway in adult T-cell leukemia/lymphoma
- Ongoing studies of role of PKCβ and IRF4
- Proximity analyses of PKCβ
- CRISPR/Cas9 PKCβ gene
- IRF4 CUT&RUN Sequencing
- CRISPRa Screen for IRF4 bypass
HTLV Vaccine
- HTLV env mRNA vaccine prevents infection in rabbits
- Efficacy correlates with nAb response
Transcriptomic Analysis of KSHV-Associated Tumors
- Single cell RNA seq of KS
- Define role of immunotherapy
